Method for fabricating stable-isotope-labeled targ

专利名称：Method for fabricating stable-isotope-labeled target peptide fragment in massspectrometry

发明人：Sumio Ohtsuki,Tetsuya Terasaki申请号：US14233592申请日：20120618公开号：US09163276B2公开日：20151020

摘要：An object of the present invention is to provide a method for producing a stableisotope-labeled target peptide fragment in mass spectrometry, which achievesinexpensive and convenient production. As a solution to attain the object, the stableisotope-labeled target peptide fragment in mass spectrometry is produced using amethod comprising the steps of: expressing a DNA conjugate in a system having a stableisotope-labeled amino acid to thereby prepare a stable isotope-labeled protein, whereinthe DNA conjugate comprises: a tandemly linked DNA in which two or more DNAsencoding one or more types of target peptide fragments are linked in tandem; and aDNA encoding a peptide fragment for concentration measurement; subjecting the stableisotope-labeled protein to fragmentation treatment with trypsin to prepare a stableisotope-labeled peptide fragment for concentration measurement and stable isotope-labeled target peptide fragments; quantifying the stable isotope-labeled peptidefragment for concentration measurement using a liquid chromatograph-tandem massspectrometer (LC/MS/MS); and calculating the concentration of the stable isotope-labeled target peptide fragment of each type from the quantification value of the stable

isotope-labeled peptide fragment for concentration measurement.

申请人：Sumio Ohtsuki,Tetsuya Terasaki

地址：Miyagi JP,Miyagi JP

国籍：JP,JP

代理机构：Chalker Flores, LLP

代理人：Chainey P. Singleton,Edwin S. Flores

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