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Ligation-Based Rna Amplification

2021-12-19 来源:趣尚旅游网
专利内容由知识产权出版社提供

专利名称:Ligation-Based Rna Amplification发明人:John R. Nelson,R. Scott Duthie,Rohini

Dhulipala,Gregory A. Grossmann,AnuradhaSekher

申请号:US11722089申请日:20051222

公开号:US20080003602A1公开日:20080103

专利附图:

摘要:Methods of amplification, purification and detection of nucleic acid sequencesespecially RNA are described. One aspect of the method involves the hybridisation and

subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to bemanipulated. The methods require that the nucleic acid structure comprises a doublestranded region and a single stranded region. The single stranded region is

complementary to the RNA sequence of interest. The double stranded region may alsocontain additional functionalities which are then used subsequently in the method.

申请人:John R. Nelson,R. Scott Duthie,Rohini Dhulipala,Gregory A.Grossmann,Anuradha Sekher

地址:Clifton Park NY US,Schenectady NY US,Kendall Park NJ US,Halfmoon NYUS,Niskayuna NY US

国籍:US,US,US,US,US

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