专利名称:Ligation-Based Rna Amplification发明人:John R. Nelson,R. Scott Duthie,Rohini
Dhulipala,Gregory A. Grossmann,AnuradhaSekher
申请号:US11722089申请日:20051222
公开号:US20080003602A1公开日:20080103
专利附图:
摘要:Methods of amplification, purification and detection of nucleic acid sequencesespecially RNA are described. One aspect of the method involves the hybridisation and
subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to bemanipulated. The methods require that the nucleic acid structure comprises a doublestranded region and a single stranded region. The single stranded region is
complementary to the RNA sequence of interest. The double stranded region may alsocontain additional functionalities which are then used subsequently in the method.
申请人:John R. Nelson,R. Scott Duthie,Rohini Dhulipala,Gregory A.Grossmann,Anuradha Sekher
地址:Clifton Park NY US,Schenectady NY US,Kendall Park NJ US,Halfmoon NYUS,Niskayuna NY US
国籍:US,US,US,US,US
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